Memory precursor phenotype of CD8 T cells reflects early antigenic experience rather than memory numbers in a model of localized acute influenza infection

Abstract

The mechanistic basis of memory T-cell development is poorly defined. Phenotypic markers that define precursors at effector stages have been characterized for acute systemic infections with high antigen load. We asked whether such markers can identify memory precursors from early effectors (d6) to late memory (>d500) for two immunodominant CD8+ responses during the course of a localized low-load influenza infection in mice. CD8+ T cells stained with the DbNP366 and DbPA224 tetramers were characterized as IL-7Rαhi, IL-7RαhiCD62Lhi or IL-7RαhiKLRG1lo. While the DbNP366- and DbPA224-specific responses were comparable in size, decay kinetics and memory precursor frequency, their expansion charac..